In vivo Measurement of the Mouse Pulmonary Endothelial Surface Layer

The endothelial glycocalyx is a layer of proteoglycans and associated glycosaminoglycans lining the vascular lumen. In vivo, the glycocalyx is highly hydrated, forming a substantial endothelial surface layer (ESL) that contributes to the maintenance of endothelial function. As the endothelial glycocalyx is often aberrant in vitro and is lost during standard tissue fixation techniques, study of the ESL requires use of intravital microscopy. To best approximate the complex physiology of the alveolar microvasculature, pulmonary intravital imaging is ideally performed on a freely-moving lung. These preparations, however, typically suffer from extensive motion artifact. We demonstrate how closed-chest intravital microscopy of a freely-moving mouse lung can be used to measure glycocalyx integrity via ESL exclusion of fluorescently-labeled high molecular weight dextrans from the endothelial surface. This non-recovery surgical technique, which requires simultaneous brightfield and fluorescent imaging of the mouse lung, allows for longitudinal observation of the subpleural microvasculature without evidence of inducing confounding lung injury.     

Zum 70. Geburtstag Von Professor Dr. Habil. Theo Wetzel

Information on the structure of the population of Phomopsis species in Ukraine, that describes the structure of Phomopsis spp. cultivated on medium: the morfological characteristics, properties of pathogenicity and phytotoxicity. It is described the method of evaluation and selection of unsusceptible forms of sunflower to phomopsis. It includes the optimal infection level, the forms of an inoculum that is effective and optimal way for artificial infection.     

Auswirkungen des Anbaus einer Wirts- und einer Nichtwirtspflanze unter wechselnden ökologischen Bedingungen auf die Populationsdynamik von Rhizoctonia solani Kühn und Fusarium solani f. pisi (Jones) Snyd. et Hans

An isometric virus was isolated from Helianthus annuus L. plants showing a yellow leaf spot mosaic on affected leaves. Infected plants were found in different ecological regions of Ukraine. A procedure of virus purification is described. The diametres of the virus particles were nonuniform and ranged from 50 to 120 nm. The sedimentation coefficient of the virus was 518–540 S and the floating density in the CsCl gradient was 1.22 g/cm³. The MW of proteins separated by electrophoresis amounted to 78±0.9, 58±0.8, 52±0.2, and 27±0.8 kDa, respectively. The virus was assigned to the tospoviruses for which sunflower is a new previously undescribed natural host plant.     

Kontinuierliche Atmungsmessungen an Azotobacter chroococcum Beij. in Montmorillonit unter chronischer Röntgenbestrahlung

Zusammenfassung An zwei Stämmen Azotobacter chroococcum Beij. wurden in Montmorillonit als Bodenmodell Atmungsmessungen unter Röntgenbestrahlung durchgeführt. Durch die Bestrahlung wurde bei beiden Stämmen die Atmungsintensität erniedrigt. Aus dem Beginn der Atmungsdepression nach Einsetzen der Bestrahlung lassen sich Rückschlüsse auf die Strahlenresistenz der verschiedenen Stämme ziehen. Die Strahlenresistenz der beiden untersuchten Bakterien-Stämme war im Montmorillonit im Vergleich zu Suspensionen wesentlich erhöht.     

An improved procedure for background correction in autoradiography

In the event of weak autoradiographic labelling, the proportion of truly labelled cells or structures can be calculated from the frequency distributions of grains per area or cell structure for i = 0, 1,..., n grains using the results obtained for an experimental group after the application of a radioactively labelled substance and those obtained for a control group without radioactivity. The principle of this computer-aided method is also applicable when the grain counts are related to varying areas in histological sections.